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Polyhydroxyalkanoate (PHA) synthase from the bacterium Bacillus cereus YB-4 (PhaRCYB4), a Class IV PHA synthase, shows both PHA polymerization and alcoholysis activities. In PhaRCYB4-expressing Escherichia coli in the presence of ethanol, biosynthesized PHA is subjected to an ethyl-esterification at the carboxy terminus as a result of alcoholytic cleavage of the PHA chain by PhaRCYB4. In this study,...
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