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Cancer gene therapy relies on nucleic acid carriers. Because of their diversity, viruses fulfill most requirements for gene delivery in clinical situations. However, parallel evolution of viruses and their hosts has made foreign protein particles, as well as infected cells, effective targets for the immune system. Although the latter consequence can be turned into therapeutic benefit, the former excludes...
The cationic polymer polyethylenimine (PEI) is a well-known non-viral delivery vector for cell transfection. In particular, the latest generation based on the linear PEI is more efficient than the first generation of branched polymers to deliver genes into mammalian cells. An overview of the properties of PEI for gene delivery will be presented here. Transient gene expression in mammalian cells...
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