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Ein bioinspiriertes Fluorophor analog zum Substrat der Glühwürmchen‐Biolumineszenz wurde hergestellt, das reagiert, wenn es schwachem blauen LED‐Licht ausgesetzt wird. Bei Lichtanregung wird dieses Material mit nahezu 81‐facher Verstärkung der Festkörperemission, Fluoreszenzquantenausbeuten von ≈90 % des Produkts in Lösung und heftigen desintegrierenden Effekten aufgrund der Freisetzung von Kohlendioxid...
Proton transfer is critical in many important biochemical reactions. The unique three‐step excited‐state proton transfer in avGFP allows observations of protein proton transport in real‐time. In this work we exploit femtosecond to microsecond transient IR spectroscopy to record, in D2O, the complete proton transfer photocycle of avGFP, and two mutants (T203V and S205V) which modify the structure of...
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