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The major diffusion channel in the outer membrane of Haemophilus influenzae type b (Hib) is porin (341 amino acids; M r 37782). The Hib porin gene was cloned and overexpressed in Bacillus subtilis. Recombinant Hib porin (Bac porin), having aggregated into inclusion bodies, was purified under denaturing conditions and subsequently refolded. To compare Bac porin that is intrinsically devoid...
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