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Ein Papierstreifen genügt ... … für die DNA‐Amplifizierung mit dem von J. D. Brennan, Y. Li et al. in ihrer Zuschrift auf S. 2759 ff. vorgestellten Verfahren. Auf ihn sind alle zum Aufbau visuell erkennbarer langkettiger DNA‐Moleküle benötigten Reagentien, wie DNA‐Polymerase, ringförmiges DNA‐Templat und kleine Bausteine, aufgebracht. Die DNA‐Amplifizierung wird durch die Zugabe einer Probe der zu...
We report on a paper device capable of carrying out target‐induced rolling circle amplification (RCA) to produce massive DNA amplicons that can be easily visualized. Interestingly, we observed that RCA was more proficient on paper than in solution, which we attribute to a significantly higher localized concentration of immobilized DNA. Furthermore, we have successfully engineered a fully functional...
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