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The hexahistidine (His 6 )/nickel(II)–nitrilotriacetic acid (Ni 2+ –NTA) system is widely used for affinity purification of recombinant proteins. The NTA group has many other applications, including the attachment of chromophores, fluorophores, or nanogold to His 6 proteins. Here we explore several applications of the NTA derivative, (Ni 2+ –NTA) 2 –Cy3. This...
The gel electrophoresis mobility shift assay is widely used for both qualitative and quantitative characterization of protein-nucleic acid interactions. Often it is found that protein-nucleic acid complexes persist within gels for much longer than would be expected on the basis of their free solution lifetimes. Excluded volume and matrix-interaction mechanisms have been proposed to account for the...
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