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The first amino acid of mature native nitrophorin 2 is aspartic acid, and when expressed in E. coli, the wild‐type gene of the mature protein retains the methionine‐0, which is produced by translation of the start codon. This form of NP2, (M0)NP2, has been found to have different properties from its D1A mutant, for which the Met0 is cleaved by the methionine aminopeptidase of E. coli (R. E. Berry,...
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