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Heterologous expression in Escherichia coli, subcellular distribution, solubility, and catalytic and substrate-binding properties of four truncated cytochromes P450 46A1 were investigated in the present study. All four lacked the N-terminal transmembrane region (residues 3-27), and, in addition, Δ46A1H had a 4x His-tag fused to the C-terminus; HΔ46A1 had the N-terminal 4x His-tag; HΔ46A1Δ had a 4x...
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