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Trichodiene synthase from Fusarium sporotrichioides contains two metal ion-binding motifs required for the cyclization of farnesyl diphosphate: the “aspartate-rich” motif D 100 DXX(D/E) that coordinates to Mg2+A and Mg2+C, and the “NSE/DTE” motif N 225 DXXSXXXE that chelates Mg2+B (boldface indicates metal ion ligands). Here, we report steady-state kinetic parameters, product array...
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