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Mutation of Thr90 to Ala has a profound effect on bacteriorhodopsin properties. T90A shows about 20% of the proton pumping efficiency of wild type, once reconstituted into liposomes. Mutation of Thr90 influences greatly the Schiff base/Asp85 environment, as demonstrated by altered λ max of 555 nm and pK a of Asp85 (about 1.3 pH units higher than wild type). Hydroxylamine...
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