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A third hydrogenase was recently identified in the proteobacterium Alcaligenes eutrophus as a constituent of a novel H 2 -sensing multicomponent regulatory system. This regulatory hydrogenase (RH) has been overexpressed in cells deficient in both the NAD + -reducing [NiFe]-hydrogenase and the membrane-bound [NiFe]-hydrogenase. EPR, FTIR and activity studies of membrane-free extracts...
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