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Complex I converts oxidoreduction energy into a proton electrochemical gradient across the inner mitochondrial or bacterial cell membrane. This gradient is the primary source of energy for aerobic synthesis of ATP. Oxidation of reduced nicotinamide adenine dinucleotide (NADH) by ubiquinone (Q) yields NAD+ and ubiquinol (QH2), which is tightly coupled to translocation of four protons from the negatively...
The spectrophotometric indicators neutral red and safranine were used to determine the relative H + /e - ratios of proton uptake from the mitochondrial matrix, and the q + /e - ratios of electrical charge translocation, during oxidation of β-OH-butyrate and succinate by ferricyanide in rat liver mitochondria. With β-OH-butyrate both ratios were higher than with succinate...
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