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Ganzkörperaufnahme: Eine selbstaktivierende 1,3‐dipolare Huisgen‐Cycloaddition und eine 6π‐Azaelektrocyclisierung von Lysin‐haltigen Dendrimeren (siehe Bild) ermöglichen die Visualisierung der In‐vivo‐Dynamik und organspezifischen Anreicherung von N‐Glycanen. Über Strukturvarianten der N‐Glycane lässt sich der Ganzkörpertransport des Clusters in Nacktmaus‐ und Krebsmodellen steuern.
We have synthesized several ganglio-oligosaccharide structures using glycosyltransferases from Campylobacter jejuni. The enzymes, α-(2→3/8)-sialyltransferase (Cst-II), β-(1→4)-N-acetylgalactosaminyltransferase (CgtA), and β-(1→3)-galactosyltransferase (CgtB), were produced in large-scale fermentation from Escherichia coli and further characterized based on their acceptor specificities. 2-Azidoethyl-glycosides...
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