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Since atrial natriuretic factor (ANF) inhibits angiotensin II (ANGII)-induced mesangial cell contraction, we studied its effect on cytosolic free calcium in fura-2-loaded monolayers. ANF lowered basal calcium from 117 +/- 5 to 89 +/- 2 nM, and also reduced the ANGII-induced calcium transient. The absolute level of cytosolic calcium after ANGII was lower in cells preincubated with ANF compared to buffer...
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