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We have developed a simple and rapid method for the purification of poly(A) tail–messenger RNA (mRNA) from total RNA by using a solid phase extraction column filled with a small amount of octadecylsilyl silica. The method is based on a hydrophobic interaction between the poly(A) tail and the octadecyl unit on the silica particle in a water/dimethyl sulfoxide mixed solution. By using this column, mRNA...
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