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Aspergillus oryzae isoprimeverose‐producing oligoxyloglucan hydrolase (IpeA) releases isoprimeverose units (α‐d‐xylopyranosyl‐(1→6)‐d‐glucose) from the non‐reducing end of xyloglucan oligosaccharides and belongs to glycoside hydrolase family 3. In this paper, we report the X‐ray crystal structure of the IpeA complexed with a xyloglucan oligosaccharide, (XXXG: Glc4Xyl3). Trp515 of IpeA plays a critical...
Carbohydrate esterase catalyzes the de-O or de-N-acylation of substituted saccharides in plant cell walls and thus has great potential for industrial biomass saccharification. We recently identified the putative carbohydrate esterase family 3 (CE3) from Talaromyces cellulolyticus. Here, we prepared the recombinant catalytic domain of the enzyme and crystallized it. The crystal structure was determined...
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