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An expression vector for purple bacteria based on the puf promoter from Rhodobacter sphaeroides was constructed. The 1.2 kb promoter fragment included the pufQ gene, ORFK, as wellas the SD sequence and thestartcodon from pufB. Translational pufduc fusion was constructed toanalyze transcription from the puf promoter. The luciferase expression was negligible under aerobic light conditions and was stimulated...
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