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ScoMcrA is a type IV modification‐dependent restriction endonuclease found in the model strain Streptomyces coelicolor. Unlike type I, II and III restriction endonucleases, which cleave unmodified DNA, type IV restriction endonucleases cleave modified DNA, including methylated, hydroxymethylated, glucosyl‐hydroxymethylated and phosphorothioated DNA. ScoMcrA targets both Dcm‐methylated DNA and phosphorothioated...
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