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The pulA gene enconding a thermostable pullulanase of 844 amino acids was cloned by PCR amplification from the genomic DNA of Thermotoga maritima MSB8. We successfully expressed the pulA gene in a protease-deficient strain Bacillus subtilis WB600, and the expressed pullulanase was secreted into the culture broth. Preliminary results showed that the pullulanase exhibited optimal activity at 90°C, and...
Due to the important physiological functions of GABA, various methods used to produce it. Biotransformation by microorganism cells is a more promising method than others. Among the GABA-producing lactic acid bacteria, one strain with high producing capacity was isolated from Chinese naturally pickled vegetable. The strain is identified as Lactobacillus brevis, and named as L.brevis TCCC13007 based...
Bacillus coagulans TL-4 strain is a thermophilic bacterium for high L-lactate production. We reported here that its 779-bp partial NAD+-dependent L-lactate dehydrogenase (LDH) gene sequence (GenBank accession no. GU354320) encoding a deduced 259 amino acid peptide was cloned by using one pair of designed degenerate primers. The amino acid sequence alignment and the built phylogenetic tree revealed...
In this study, the 5' terminus of ldhL gene in Lactobacillus casei ATCC 334 was fused to the 3' terminus of pdc gene promoter (Ppdc) part in Zymomonas mobilis ATCC 10988 by recombinant PCR. The fusion gene Ppdc-ldhL was integrated in broad-host-range plasmid pBBR1MCS3, transformed by electroporation and expressed in Z. mobilis ATCC 10988. The primary expression of heterologous lactate dehydrogenase...
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