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This paper reports a new integrated microfluidic platform capable of automating two kinds of on-chip tissue slide-based in-vitro screening processes, including a systematic evolution of ligands by exponential enrichment (SELEX) process and a phage display screening process. Tissue slide is one of the most important biopsy samples for cancer patients in clinical diagnosis. It is the first time that...
This study presents a passive, self-driven microfluidic device which can rapidly identify influenza A (HlNl) virus by using reverse transcription loop-mediated isothermal amplification (RT-LAMP). H1N1-specific aptamer conjugated magnetic beads were used to capture virus and the RT-LAMP product could be detected by visual coloration. The entire diagnosis could be automatically performed by capillary...
In this study, a new screening process called "bacterial SELEX" was reported to screen favorable aptamers targeting four common nosocomial and antibiotics-resistant bacteria, including Acinetobacter baumannii, vancomycin-resistant Enterococci (VRE), Escherichia coli, and multidrug-resistant Staphylococcus aureus (MRSA). The new bacterial SELEX system performed three critical selecting stages...
This study reported an integrated microfluidic system for automating multiplex detection of allergy microarrays. Allergy has become a common disease worldwide. However, detection for allergy on microarrays requires tedious manual operation, well-trained technicians and a relatively labor-intensive process. In this study, an integrated microfluidic system combining the microfluidic techniques and the...
This study reports a magnetic-bead based microfluidic system to capture cholangiocarcinoma cells by using structurally well-defined heparan sulfate (HS) octasaccharides. Ten synthetic HS octasaccharides (from SCH-43 to SCH-52) were coated on magnetic beads for this study, respectively. An integrated microfluidic system equipped with micromixers, normally-closed valves, and microchambers was developed...
this study presents a simple microfluidic device which can perform sample pretreatment automatically for rapid bacteria detection. Herein, a new approach to rapidly isolate bacteria incorporated with vancomycin-conjugated magnetic beads by using a new membrane-type microfluidic component with a floating block was proposed and its performance was tested and verified. The experimental results showed...
In this work, we proposed a new method using optically-induced dielectrophoresis (ODEP) and optically-induced localized electric field (OILEF) to realize precise cellpairing and cell fusion automatically on a structure-free optoelectronic device. “Virtual” electrodes, which were generated by specially designed light patterns, were used for cell-pairing and cell fusion. After pairing cells by using...
Enzyme-linked immunosorbent assay (ELISA) is a popular detection method which employs two antibodies. In this study, we presented a new method which used electromagnetically-driven microdevices to form an integrated microfluidic chip for automating ELISA-like dual aptamer assay. First, an electromagnetic micropump was designed and fabricated by using composite membranes. It was formed by mixing iron...
A simple microfluidic device which can perform antimicrobial susceptibility testing (AST) of combined antibiotics against antibiotics-resistant bacteria has been presented. A new approach to determine the fractional inhibitory concentration (FIC) index of two antibiotics by performing broth dilutions automatically was proposed and its performance was highly consistent with manual operation. The experimental...
This study presents an integrated microfluidic system combining two techniques, including an optically-induced dielectrophoresis (ODEP) module for manipulation of drug particles and an UV-direct-writing module capable of patterning of hydrogel, for applications of digital drug cocktails. This system was able to provide an automatic and customized production of drug cocktails. Experimental results...
A high-sensitivity biosensor is demonstrated by exploiting surface plasma (SP) enhanced transverse magneto-optical Kerr effect (TMOKE) on a packaged micro-optofluidic chip to implement a compact system for detecting chemical or biological objects. The SP grating made on a composite Au/Fe/Au layer exhibits a very dispersive Kerr parameter variation near the SP resonance wavelength. A preliminary experimental...
Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique which amplifies DNA with high specificity, efficiency and rapidity under isothermal conditions. In this study, a microfluidic droplet-array chip is presented to implement array-based digital LAMP analysis. The proposed microfluidic system can perform the basic operation of droplets including droplet formation,...
Digital loop-mediated amplification (LAMP) is an isothermal, quantitative DNA amplification approach which has high sensitivity and specificity and could be used to analyze extremely low amount of DNA within a short period of time. In this study, an integrated self-driven microfluidic chip was proposed herein for performing a digital LAMP process for vancomycin-resistant Enterococcus (VRE) diagnosis...
Cholangiocarcinoma (CCA) is a cancer of bile duct, which possesses a high mortality rate and poor prognosis owing to its difficulties in early diagnosis and a lack of effective treatment. To improve the clinical detection, one of promising approaches is to find specific biomarkers to detect CCA cells. However, the existing screening processes are usually time-consuming and lab-intensive. In this study,...
Rapid and accurate diagnosis of C-reactive protein (CRP) is crucial for monitoring cardiovascular diseases because it is a well-known biomarker for evaluating risks of cardiovascular diseases. This study presents a dual-aptamer assay for detection of CRP by using field-effect transistors (FET). This is the first time that two aptamers, which are specific to CRP, were used to form a sandwich assay...
This study presents an integrated microfluidic system which can identify the vancomycin resistant gene (vanA) from live hetero-bacteria automatically. In this study, a new approach to diagnose the vanA gene from live hetero-bacteria by using ethidium monoazide (EMA) and loop-mediated isothermal amplification (LAMP) was proposed and its feasibility was tested and verified. In addition, an integrated...
A technique using digital masks without ultraviolet light to rapidly print 3-D biopolymer structures with complex microarchitectures in a microfluidic chip has been demonstrated. In this approach, a customized system is used to project light images on a photoconductive substrate in order to create localized virtual electrodes when an alternating electric field is applied across the fluidic medium...
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