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In vitro synthesized mRNAs are often used as calibrants in gene expression analysis. In the case of an analytical procedure for gene expression containing a process of reverse transcription such as microarray analysis, full-length mRNAs having a 3′-poly(A)+tail are required as calibrants. However, the preparation of full-length mRNAs having a 3′-poly(A)+tail by using ordinary plasmid vectors is difficult...
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