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Cultivation of mammalian cells holds promise for generating cell and tissue models and transplants, but the shortcomings of current cell culture methods are limiting such use. In order to provide biological material that can represent cells and tissues of the body in drug development and regenerate organs and systems impaired by disease in the clinic, it is important to recognize that the extracellular...
For the purpose of corneal regenerative medicine, we fabricated human corneal endothelial cell sheets on temperature-responsive dishes, which could be non-invasively harvested as intact, transplantable sheets by simply reducing the culture temperature. Cells demonstrated hexagonal cell shape with numerous microvilli and cilia, and also exhibited abundant cytoplasmic organelles similar to these cells...
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