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PTEN is a dual protein and lipid phosphatase that dephosphorylates PIP3 at the 3 ' position, thereby antagonizing PI3-kinase activity. A reduction in PI3 ' kinase activity enhances the susceptibility of cells to apoptosis. By stably transfecting PC12 cells with an antisense PTEN construct, endogenous PTEN protein levels were reduced by ~50% and etoposide-induced apoptosis was markedly...
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