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In the frame of protein identification from mouse adipose tissue, two strategies were compared for the offline elution of peptides from a strong cation exchange (SCX) column in two-dimensional liquid chromatography tandem mass spectrometry (2D–LC–MS/MS) analyses. First, the salt gradient (using K+ as displacing agent) was evaluated from 25 to 500mM KCl. Then, a less investigated elution mode using...
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