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To measure protein isoforms in individual mammalian cells, we report single‐cell resolution isoelectric focusing (scIEF) and high‐selectivity immunoprobing. Microfluidic design and photoactivatable materials establish the tunable pH gradients required by IEF and precisely control the transport and handling of each 17‐pL cell lysate during analysis. The scIEF assay resolves protein isoforms with resolution...
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