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Pore‐gradient microgel arrays enable thousands of parallel high‐resolution single‐cell protein electrophoresis separations for targets accross a wide molecular mass (25–289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels facilitate gel‐pore expansion after electrophoresis for efficient and uniform immunoprobing. The photopatterned, light‐activated, and acid‐expandable hydrogel...
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