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The complementary DNA (cDNA) sequence of Gly m Bd 28K was expressed in Escherichia coli BL21 (DE3) as an inclusion body under the induction of 1.0 mmol/L of isopropyl β-D-thiogalactoside (IPTG). The inclusion bodies were solubilized in 6 M guanidine hydrochloride and refolded by rapid dilution. After renaturation, the purity of the recovered recombinant protein was up to 90 %, and its molecular weight...
This paper presents the generation of monoclonal antibodies (mAbs) with high specificity against benzopyrene (BaP) through cell fusion procedures, and the development of mAb-based indirect competitive enzyme-linked immunoabsorbent kit (icELISA kit) to detect BaP using one of these hybridomas (clone 2E12). The calibration curve of BaP-Kit with standard BaP inhibitor was typical sigmoid curve fitted...
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