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Mouse lens cultures were employed to study the progression of cataracts initiated by injection of buthionine sulfoximine, an inhibitor of glutathione (GSH) biosynthesis. Culture of lenses removed from untreated mice on postnatal day 7, for 48 hr in the presence of 4 mmBSO, resulted in only limited cataractous changes. To enable substantial progression of cataracts in vitro, it was therefore necessary...
Cataract induction in preweanling mice byl-buthionine sulfoximine (BSO), an inhibitor of glutathione biosynthesis, was correlated with perturbations in vitro protein biosynthesis. These were detected by incubation of late precataract and early cataract lenses with 35 S-labeled amino acids, followed by dissection of lenses into capsule-epithelium and decapsulated fiber fractions, further...
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