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An amperometric culture-based method was developed for rapid detection of viable Escherichia coli in water. The bacteria were recovered by filtration and incubated in a selective medium, lauryl sulphate broth (LSB) supplemented with the substrate 4-aminophenyl-β-d-galactopyranoside (4-APGal) at 44.5 o C. The electrochemically active molecule 4-aminophenol (4-AP) was produced after hydrolysis...
An amperometric detection system for horseradish peroxidase (HRP) activity was optimized using flow injection analysis (FIA) with glassy carbon as a working electrode. Ferroceneacetic acid was investigated as a co-substrate for the electrochemical detection of HRP. The calculated detection limit for HRP was 2.6 10 -12 M with incubation of 30 min. The substrate was used in an...
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