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The presence of a trifluoromethyl group has implications on the enantioselective biotransformation of ethyl 4,4,4-trifluoro-acetoacetate with Saccharomyces cerevisiae (Baker’s yeast). Reaction of the trifluoro-keto ester with the aqueous solvent leads to hydration of the carbonyl group and reduces bioavailability of the substrate. The hydrate forms an equilibrium with the enol and the keto substrate...
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