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Reconstitution of factor VIII from isolated heavy chain (HC) and light chain (LC) shows pH-dependence. In the presence of Ca 2+ , up to 80% of native factor VIII activity was recovered over a wide range of pH. In contrast, affinity of HC and LC was maximal at pH 6.5–6.75 (K d ∼4 nM), whereas a K d ∼20 nM was observed at physiological pH (7.25). The effect of Cu 2+ (0...
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