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To evaluate the physiological effect and response to noxious stimulation at five concentrations of MS-222 in koi (Cyprinus carpio). Prospective experimental study. Twenty-one healthy adult unknown sex koi fish weighing mean 450 ± SD 120 g. Each fish was exposed to five different concentrations of MS-222 (50, 70, 110, 150 and 190 mg L−1) in a random sequence during the same anaesthetic event...
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