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A novel chitinase gene (PbChi70) from a marine bacterium Paenicibacillus barengoltzii was cloned and functionally expressed in Escherichia coli. The recombinant enzyme (PbChi70) was purified to homogeneity with a recovery yield of 51.9%. The molecular mass of purified enzyme was estimated to be 70.0kDa by SDS–PAGE. PbChi70 displayed maximal activity at pH 5.5 and 55°C, respectively. It exhibited strict...
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