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Various native starch granules were reacted as substrates for a debranching enzyme, pullulanase, from Bacillus acidopullulyticus, and the kinetic mechanism was investigated in initial rate studies. The apparent Km values of the native starch hydrolysis reactions decreased in the order of rice, corn, and potato, whereas Vmax remained constant for all starches. Assuming that starch granules were spherical,...
Rice starch was subjected to enzymatic treatment with pullulanase and the physicochemical properties of the modified rice starch were investigated as a function of the degree of hydrolysis which ranged from 0.9 to 10.2%. The enzymatic hydrolysis of rice starch caused a decrease in the fraction of rapidly digestible starch (RDS) (78.31→44.65%), whereas the levels of slowly digestible starch (SDS) (0...
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