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A multiplex fluorescence signal amplification method based on microchip electrophoresis (MCE) platform was developed for the improvements in the separation and detection of microRNAs. The method used two kinds of fluorescein amidite labeled DNA signal probes to hybridize with its target microRNAs, utilizing T7 exonuclease assisting target circling realized the fluorescence signal amplification. Then,...
Glutathione (GSH) is a major endogenous antioxidant that has a central role in cellular defense against toxins and free radicals. Rapid and accurate detection of GSH content in single cells is important to the early diagnosis of disease and biomedical research. In this work, a novel method based on microchip electrophoresis chemiluminescence (MCE-CL) detection was developed for the quantification...
A sensitive immunoassay method based on microchip electrophoresis chemiluminescence (MCE-CL) detection technology was developed for the detection of tumor marker alpha-fetoprotein (AFP). This method adopts the non-competitive immunoassay mode, and was conducted after AFP reacted with excessive horseradish peroxidase (HRP) labeled monoclonal antibody. The extreme pH value was adopted in the electrophoresis...
A novel sulfur and nitrogen binary doped carbon dots (S,N-CDs) was synthesized by one-step manner through the hydrothermal treatment of citric acid (CA) and ammonium thiocyanate, and the procedures for biomedical applications, including probing doxycycline in living cells and multicolor cell imaging were developed. The obtained S,N-CDs are stable in aqueous solution, possess a very high quantum yield...
Nitrogen doping has been a powerful method to modulate the properties of carbon materials for various applications, and N-doped graphene quantum dots (GQDs) have gained remarkable interest because of their unique chemical, electronic, and optical properties. Herein, we introduce a facile one-pot solid-phase synthesis strategy for N-doped GQDs using citric acid (CA) as the carbon source and 3,4-dihydroxy-l-phenylalanine...
An enhanced chemiluminescence resonance energy transfer (CRET) system based on target recycling G-guadruplexes/hemin DNAzyme catalysis was developed for ultrasensitive detection of DNA. CRET system consists of luminol as chemiluminescent donor, and fluorescein isothiocyanate (FITC) as acceptor. The sensitive detection was achieved by using the system consisted of G-riched DNA, blocker DNA, and the...
Bovine serum albumin stabilized Au nanoclusters (BSA–AuNCs) were demonstrated as a novel fluorescence probe for sensitive and selective detection of free chlorine in drinking water. The fluorescence of BSA–AuNCs was found to be quenched effectively by the free chlorine, and the decrease in fluorescence intensity of BSA–AuNCs allowed the sensitive detection of free chlorine in the range of 0.8–800μM...
A signal amplification strategy based on antibody–gold nanoparticle–DNAzyme assembly in capillary electrophoresis based chemiluminescent immunoassays (CE-CLIA) was developed. In this CE-CLIA, antibody–AuNP–G-quadruplex/hemin was incubated with limited amount of antigen, and the formed immunocomplex and unreacted antibody–AuNP–G-quadruplex/hemin were then separated by CE and detected by CL. Due to...
A novel nano-adsorbent, Fe 3 O 4 @ionic liquid@methyl orange nanoparticles (Fe 3 O 4 @IL@MO NPs), was prepared for magnetic solid-phase extraction (MSPE) of polycyclic aromatic hydrocarbons (PAHs) in environmental water samples. The Fe 3 O 4 @IL@MO NPs were synthesized by self-assembly of the ionic liquid 1-octadecyl-3-methylimidazolium bromide (C18mimBr)...
A highly sensitive and selective multi-walled carbon nanotube (MWCNT)-based multicolor fluorescent nanobeacon is developed for multiplexed analysis of DNA in homogeneous solution. In this work, three different dye-labeled DNA hairpins were adsorbed on MWCNTs surface via π-stacking, which brings the dyes and MWCNTs into close proximity and leads to the quenching of fluorescence of the dyes. When target...
A multicolor quantum dot (QD)-based nanosensor for multiplex detection of two tumor markers in a homogeneous format based on fluorescence polarization immunoassay was proposed. QDs520 and QDs620 were labeled alpha-fetoprotein(α-AFP) and carcinoembryonic antigen (CEA), respectively. After separated and purified by ultrafiltration, they were used in fluorescence polarization immunoassay for the simultaneous...
A sensitive method based on quantum dot (QD)-enhanced capillary electrophoresis–chemiluminescence (CE–CL) detection was developed for simultaneous determination of dopamine (DA) and epinephrine (E). In this work, CdTe QD was added into the running buffer of CE to catalyze the post-column CL reaction between luminol and hydrogen peroxide, achieving higher CL emission. Negative peaks were produced due...
A novel strategy for the preparation of in-column adenosine deaminase (ADA) microreactor and rapid screening of enzyme inhibitors in natural extracts was demonstrated. In this approach, ADA was encapsulated in anionic polyelectrolyte alginate that was immobilized on the surface of fused-silica capillary via ionic binding technique with cationic polyelectrolyte polyethylenimine (PEI). On-line enzyme...
A rapid and simple method using capillary electrophoresis (CE) with chemiluminescence (CL) detection was developed for the determination of levodopa. This method was based on enhance effect of levodopa on the CL reaction between luminol and potassium hexacyanoferrate(III) (K 3 [Fe(CN) 6 ]) in alkaline aqueous solution. CL detection employed a lab-built reaction flow cell and a photon...
A capillary electrophoresis method has been developed for the determination of d-serine in neural samples. d/l-serine was tagged with naphthalene-2,3-dicarboxaldehyde (CBI-d/l-Ser), and the separation of CBI-d/l-Ser enantiomer was achieved by using a dual chiral selector system consisting of β-cyclodextrin (β-CD) and chiral micelles formed by sodium deoxycholate (SDC). No resolution was observed when...
A flow-injection method for the simultaneous determination of iron and zinc in the human hair with 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol (5-Br-PADAP) using a pH gradient technique has been developed. The linear range for the determination of iron is 0.1∼1.8 μg ml −1 and for zinc is 0.2∼5.0 μg ml −1 . About 20∼30 samples can be determined in 1 h. The proposed method is simple,...
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