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We have utilized DSC and high pressure FTIR spectroscopy to study the specificity and mechanism by which ATP protects actin against heat and pressure denaturation. Analysis of the thermograms shows that ATP raises the transition temperature Tm for actin from 69.6 to 75.8 o C, and the calorimetric enthalpy, ΔH, from 680 to 990 kJ/mole. Moreover, the peak becomes sharper indicating a more cooperative...
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