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It is widely accepted that the vesicle-transporter myosin-V moves processively along F-actin with large steps of ∼36nm using a hand-over-hand mechanism. A key question is how does the rear head of two-headed myosin-V search for the forward actin target in the forward direction. Scanning probe nanometry was used to resolve this underlying search process, which was made possible by attaching the head...
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