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The variation in mobile phase pH and ionizable solute dissociation constant (pK a ) with the change of organic modifier fraction in hydroorganic mobile phase has seemingly been a troublesome problem in studies and applications of reversed phase high performance liquid chromatography (RP-HPLC). Most of the early studies regarding the RP-HPLC of acid–base compounds have to measure the actual...
The utilization of neutral compounds as model compounds is put forward for determination of the n-octanol/water partition coefficient (K ow ) of highly hydrophobic, weak acidic compounds by reversed-phase high performance liquid chromatography (RP-HPLC). It is based on a linear relationship between the logarithm of apparent n-octanol/water partition coefficient (logK ow ″), expressing...
n-Octanol/water partition coefficients (P) for DDTs and dicofol were determined by reversed-phase high performance liquid chromatography (RP-HPLC) on a C 18 column using methanol–water mixture as mobile phase. A dual-point retention time correction (DP-RTC) was proposed to rectify chromatographic retention time (t R ) shift resulted from stationary phase aging. Based on this correction,...
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