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Hepatocytes cultured as multicellular aggregates called spheroids exhibit enhanced liver functions and maintain them over a long period compared with monolayer culture. We previously reported the induction of hepatocyte spheroids using the synthetic polymer Eudragit (a copolymer of methacrylic acid and methylmethacrylate) as an artificial matrix in a cell suspension system (Yamada et al., J. Biochem...
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