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Ten polymorphic microsatellite loci were isolated for Physalaemus cuvieri from a GA—CA enriched library. In 160 P. cuvieri individuals, the number of alleles per locus ranged to 2–9 and the expected heterozygosity ranged from 0.30 to 0.85. The primers were successfully cross-amplified in the congeneric species P. albonotatus, P. ephippifer and Physalaemus cf. cuvieri, suggesting that these loci are...
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