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Objective: Seeding and patterning of cells with an engineered scaffold is a critical process in artificial tissue construction and regeneration. To date, many engineered scaffolds exhibit simple intrinsic designs, which fail to mimic the geometrical complexity of native tissues. In this study, a novel scaffold that can automatically seed cells into multilayer honeycomb patterns for bone tissue engineering...
This paper presents a new technique of fabricating 3D-printed scaffolds that can utilizes dielectrophoresis (DEP) for cell patterning. The scaffold was first fabricated using a 3D printer with a biodegradable polymer, polylactic acid (PLA). The electrical conductivity of the polymeric scaffold was enhanced through sputtering a thin layer of gold. When a voltage was supplied to the scaffold, non-uniform...
Dielectrophoresis (DEP) has widely been used for manipulation and patterning of biological cells. In this paper, a novel multi-layer scaffold structure was designed for patterning cells in 3D via dielectrophoresis. Honeycomb patterns were integrated in each layer of the structure in order to pattern cells into bone-like tissues. When a voltage was supplied to the scaffold structure, non-uniform electric...
Automatic manipulation and patterning of biological cells into an artificial scaffold is an imperative step in the production of high-quality tissue for tissue transplantation. This paper examines the incorporation of dielectrophoresis into a three-dimensional (3D) scaffold body for batch manipulation and patterning of cells. To facilitate dielectrophoresis-based manipulation, a multi-layer biocompatible...
Patterning and assembly of biological cells in a 3D structure represents an important process in artificial tissue engineering. This paper presents the design of a multi-layer electrode scaffold used to assemble biological cells into a 3D pattern. Through suppling voltage to the multi-layer scaffold, three-dimensional electric fields can be established to manipulate cells towards the scaffold via...
This paper presents a microfluidic chip design that utilizes dielectrophoresis (DEP) tweezers to capture and stretch cells while measuring Raman spectra of cells under different deformations. The microfluidic chip is made of PDMS and glass slide bonded. The DEP force is generated that drives the cells to pass through the required area successively. A novel microelectrode with DEP tweezers is designed...
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