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In order to investigate the potential mechanisms in troglitazone-induced apoptosis in HT29 cells, the effects of PPARγ and POX-induced ROS were explored. [3- (4, 5)-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay, Annexin V and PI staining using FACS, plasmid transfection, ROS formation detected by DCFH staining, RNA interference, RT-PCR & RT-QPCR, and Western blotting analyses...
Both of ceramide and PPARγ ligand can trigger cancer cell apoptosis. We here show that C2-ceramide can modulate PPARγ expression level and its transcriptional activity and results in apoptosis in HT29 cells. Administration of PPARγ specific antagonist GW9662 partially prevents HT29 cells from apoptosis. Furthermore, MAP kinase pathway provided a potential modulation mechanism for PPARγ pathway related...
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