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The identity of the histidine specific transfer RNA (tRNA His ) is largely determined by a unique guanosine residue at position −1. In eukaryotes and archaea, the tRNA His guanylyltransferase (Thg1) catalyzes 3′–5′ addition of G to the 5′-terminus of tRNA His . Here, we show that Thg1 also occurs in bacteria. We demonstrate in vitro Thg1 activity for recombinant enzymes from...
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