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S. aureus USA300 isolates utilize the copBL and copAZ gene products to prevent Cu intoxication. We created and examined a ΔcopAZ ΔcopBL mutant strain (cop‐). The cop‐ strain was sensitive to Cu and accumulated intracellular Cu. We screened a transposon (Tn) mutant library in the cop‐ background and isolated strains with Tn insertions in the mntABC operon that permitted growth in the presence of Cu...
Glutathione (GSH) is an abundantly produced low-molecular-weight (LMW) thiol in many organisms. However, a number of Gram-positive bacteria do not produce GSH, but instead produce bacillithiol (BSH) as one of the major LMW thiols. Similar to GSH, studies have found that BSH has various roles in the cell, including protection against hydrogen peroxide, hypochlorite and disulfide stress. BSH also participates...
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