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Cross-linked tyrosinase aggregates were prepared by precipitating the enzyme with ammonium sulfate and subsequent cross-linking with glutaraldehyde. Both activity and stability of these cross-linked enzyme aggregates (CLEAs) in aqueous solution, organic solvents, and ionic liquids have been investigated. Immobilization effectively improved the stability of the enzyme in aqueous solution against various...
Ionic liquids (ILs) have shown potentials as a new reaction medium for biocatalysis, either as such or as an aqueous mixture. Their unique ionic nature is important in affecting the enzyme performance, and the Hofmeister effects provide a reasonable explanation for this. In this review, the impact of Hofmeister effects on enzyme performance in aqueous solution was first examined, concerning the specific...
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