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The aim of this work was to compare a validated liquid chromatography–mass spectrometry (LC–MS) method with the commercial enzyme multiplied immunoassay technique (EMIT) for cyclosporine and tacrolimus whole blood quantification.Samples of transplant patients receiving cyclosporine (n=38) or tacrolimus (n=41) were analyzed successively by LC–MS and EMIT. Several statistical approaches for method comparison...
The aim of this work was to develop a selective method for the simultaneous quantification of cyclosporine, tacrolimus, sirolimus and everolimus in whole blood.An automated on-line solid-phase extraction system coupled with liquid chromatography–mass spectrometry (LC–MS) was used. After a simple protein precipitation, the supernatant was load on a C8 column with a mobile phase composed of MeOH/H ...
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