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A recombinant plasmid, pRP-GFP, harboring the green fluorescent protein (GFP) gene from the broad-host-range mobilizable plasmid pRK415 of the RK2 family was constructed and transferred by conjugation from Escherichia coli S17-1 to Sorangium cellulosum So ce90. The results of Southern blot analysis showed that the plasmid replicated autonomously without being integrated into the chromosome of the...
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