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A fluorogenic model substrate, methylumbelliferyl-N-acetylglucosamine was used to measure N-acetylglucosaminidase activities in wetland soils. The enzyme activity exhibited substrate saturation around 200 μM, and an approximate linearity for at least 90 min incubation time. The effects of temperature, pH, toluene, and γ-radiation on the enzyme were also determined. The method was applied to 32 different...
A fluorogenic model substrate, methylumbelliferyl-N-acetylglucosamine was used to measure N-acetylglucosaminidase activities in wetland soils. The enzyme activity exhibited substrate saturation around 200 μM, and an approximate linearity for at least 90 min incubation time. The effects of temperature, pH, toluene, and γ-radiation on the enzyme were also determined. The method was applied to 32 different...
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