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BACKGROUNDBiodiesel production is not economically competitive with petroleum diesel particularly when using virgin and refined vegetable oils. Rich‐in‐oil miscella obtained from the extraction of soybean oil with ethanol may be a promising feedstock for taking off the refining process, simultaneously introducing an environmental friendly step by replacing hexane by ethanol as a renewable solvent in the oil extraction process. The aim of this study was to investigate the production of biodiesel from the oil–ethanol miscella by direct transesterification using Novozym®435 as catalyst and ethanol as acyl acceptor; simultaneously optimizing the process by response surface methodology and enzyme reuse...
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