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Aspartic protease is a versatile protease used in the food processing industry. A novel aspartic protease gene (P10) was cloned from an Antarctic psychrophilic fungus Geomyces pannorum and successfully expressed in Aspergillus oryzae when cultured at 20°C. However, purified P10 exhibited optimal activity at 60°C and retained approximately 80% activity at 50–70°C. The Km and Vmax values for this protease...
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